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Submit your Real-Time PCR questions and watch the rest of our videos at http://ow.ly/bQh0l. Life Technologies Sr. Field Application Specialist Doug Rains helps with the understanding the causes of multiple peaks in a melt curve when using SYBR® Green dye in Real-Time PCR. SYBR® Green I chemistry is a free-floating dye. Here's how it works ...

Basic temperature mapping tips that will help to achieve accurate qualification test results that will help to ensure sample safety. 0:00 Introduction 1:18 Create discovery questions 2:17 Documentation 2:51 Technical data sheet 3:27 Proper planning & purchasing 3:51 Proper probe placement 4:47 Dial in the freezer 5:38 Test failure protocol 6:39 ...

Are you using Applied Biosystems SYBR Green chemistry for your real-time experiment and getting multiple peaks in your melt curve? Let's chat about it in Taq Talk Episode 20: "Finding multiple melt-curve peaks when using SYBR Green dye in real-time PCR." For more information about using SYBR Green Master Mix in your experiments: https://www ...

Simplify downstream calculations with the Bioprocessing Downstream Calculators. Learn more about the Bioprocessing Downstream Calculators and how they can help make your biopharma downstream calculations fast, easy and efficient.

https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-enzymes-master-mixes.html?cid=BID_R01_PJT4572_COL19401_VI_YUT_OD_KT_234 Learn the importance of the annealing step in PCR, how to reduce optimization steps using a specially formulated PCR buffer, and the benefits of a universal annealing temperature enabled by the buffer. Find DNA ...

Set up and start using the Thermo Scientific™ TSX™ Core Ultra-Low Temperature Freezer by learning the basics of its user interface. 0:00 Introduction 0:38 Operating setpoint 0:51 Changing setpoint 1:12 Settings menu 1:26 Warm and cold warnings 1:43 Control offset 2:49 Security passcode 3:12 Warnings and cautions 3:44 Program menu 4:06 ...

Learn about best practices for short and long term ultra low temperature (ULT) freezer maintenance. This session will help enable maximization of energy efficiency, performance, and the reliability of your ultra low freezer investment. Topics: 0:00 Introduction and overview 0:34 What is a ULT freezer? 1:57 How does a ULT work? 2:50 Introduction ...

Learn important settings and setup information for your Thermo Scientific™ ALPS5000 Automated Plate Heat Sealer. 0:00 Introduction 0:23 Main screen 0:48 Setting setpoint temperature 1:16 Setting setpoint time 1:40 Changing setpoint mode 2:31 Settings menu 3:39 Changing mode setting 2:53 Conclusion View the 3D product tour: https://www ...

How to Measure Melting Temperature using the TM Analysis System Shimadzu’s new Tm Analysis system comprises of the UV-Vis spectrometer, the TMSPC8i temperature-controlled cell holder, and the LabSolutions UV-Vis Tm Analysis software. Determination of melting temperature for your biomolecules can be achieved with great flexibility and ...

Learn how to use the Batch calculation type in the Property Model Calculator in Thermo-Calc to calculate solidus and liquidus temperatures. The Batch calculation type allows you to upload a data file to the program so that you can perform calculations with significant amounts of input data. The calculation uses a demo database, so it can be run ...

To watch more videos, join our academy.... https://www.udemy.com/dna-primer-design-for-pcr/ Facebook page http://adf.ly/1fShHH This video explain how the Tm using Wallace equation, Marmur and Doty equation, and the salt-adjusted method. DNA primer, How to calculate tm, tm of primer, melting temperature calculation for dna, dna melting ...

This video explains about online tool for primer analysis, primer dimer and calculation of Tm. It has option to change parameters that are typically used in PCR master mix.. This can be accessed from https://eu.idtdna.com It is a great tool for oligo analysis and it is available for free Related Videos ...

Watch how easily and quickly you can predict melting temperature, dimers, hairpins, and more for any oligonucleotide design with IDT’s OligoAnalyzer™ Tool. In this official IDT video tutorial, we will show you how to use the OligoAnalyzer™ Tool to calculate the physical characteristics of oligos before you order them to you save, time ...

This chemistry video lecture tutorial focuses on thermochemistry. It provides a list of formulas and equations that you need to know as well as the appropriate units. It provides a nice review covering topics such as the internal energy of a system, the surroundings, endothermic vs exothermic processes, work, pressure, and volume. It explains ...

This video introduces you to Thermo-Calc Graphical Mode and shows you how to set up a basic calculation. After you watch this video, you can watch the examples videos to learn how to use the various calculators and modules in the software: https://www.youtube.com/playlist?list=PLfv6McToaTGSzHrh3TfNF2EhoUUUqeEWd ~~TIMESTAMPS~~ 0:00 Introduction ...

Watch how to carrying out a melting point determination. At the Royal Society of Chemistry we provide education resources via our website Learn Chemistry to enhance teaching and learning The find more laboratory technique videos view our resource: http://www.rsc.org/learn-chemistry/resource/res00001064/the-interactive-lab-primer

Submit your Real-Time PCR questions and watch the rest of our videos at http://ow.ly/bQh0l. Life Technologies Sr. Field Application Specialist Doug Rains helps with the understanding of baselines in Real-Time PCR. We're looking at a fairly standard real-time amplification plot. We have some nice curves, each of which has the familiar geometric ...

The first-ever app in the industry with personalized experience backed by data science, allowing rich discovery, custom product recommendations, and a fast way to place your orders. It provides the latest updates and access to an extensive database of articles, videos, webinars, and much more. You can directly login via your thermofisher.com ID ...

Contact Us: Phone: 608-231-1907 E-mail: info@madisongroup.com Thermogravimetric analysis (TGA) is an extremely important problem solving tool for material characterization, material identification, and failure analysis efforts. This video shows how the results of this analysis are interpreted and used by The Madison Group to solve problems in ...

Thermo-Calc comes with dozens of examples to help you learn how to use the software. This video will show you how to use the graphical mode examples for Thermo-Calc and all of the Add-on Modules. See all of the tutorial videos on our website: https://thermocalc.com/support/video-tutorials/ Read the examples manual: https://thermocalc.com ...

Hello friends, today we will discuss PCR annealing temperature. I know it's a very basic thing in molecular biology but this video will b helpful for newbies to understand what annealing temperature in PCR is? So stay tuned…. *************************************************************************************** Hello, this is Dr. Manushi ...

Thermo-Calc is software designed for materials scientists and engineers. With Thermo-Calc you can make calculations that give you information to help you make better decisions for designing and processing materials. This video gives you an overview of Thermo-Calc and what this powerful software can do for you. Learn more and download a free ...

To learn more, visit http://www.lifetechnologies.com/castpcr With the new LifeTechnologies developed castPCR technology, somatic mutations now have nowhere to hide. The TaqMan® Mutation Detection Assays use this castPCR™ technology, (which refers to competitive allele-specific TaqMan® PCR) are specifically designed to detect somatic ...

Learn how to calculate martensite transformation temperatures as well as martensite fractions for an Fe-C-Cr alloy that was intercritically annealed at two different temperatures using Thermo-Calc. The first calculation in this video shows how to calculate martensite fractions, which is the total martensite percentage of an Fe-Cr-C alloy that ...

"Real-time PCR can be challenging to learn, but it doesn’t have to be. Become a qPCR expert - watch the Taq Talk video series at https://www.thermofisher.com/taqtalk?cid=gsd_pcr_sbu_r01_co_cp1447_pjt7842_ext1253_0vi_yut_vo_awa_og_s00_ep1 While you're there, check out our handy guide for common acronyms and terms: https://www.thermofisher.com ...

This chemistry video tutorial provides a basic introduction into the equations and formulas that you need to solve common Thermochemistry practice problems. Thermochem - Free Formula Sheet: https://www.video-tutor.net/chemistry-formula-sheets.html ______________________________ First Law of Thermodynamics: https://www.youtube.com/watch?v ...

Now that we have covered some important separation techniques, let's take a look at a purification technique. Sometimes a chemical reaction will produce a solid, which can precipitate from solution. But these crystals typically contain impurities from solution that were trapped in the lattice as it formed. After we isolate these crystals, we ...

Thermogravimetric analysis (TGA) is a frequently used technique in thermal analysis. Its main use is to characterize materials by measuring change in their mass as a function of temperature or time. The TGA measurements are used mainly to determine the materials composition, thermal stabilities, oxidative stabilities, product lifetimes ...

Please consider supporting the channel on Patreon! https://www.patreon.com/SupremeScience This video demonstrates how to use the Mel-Temp to take melting points in the chemistry lab.

Annealing temperature is very crucial for carrying out successful PCR (Polymerase Chain Reaction ). The low annealing temperature always produces the non specific amplification while annealing temperature equals or more than melting temperature results in no amplification .Thus there is need to calculate annealing temperature to carry out ...

How To Calculate The Temperature? In this informative video, we will guide you through the process of calculating the temperature of an object after it has been heated. Understanding how to perform this calculation is essential for anyone interested in physics or engineering. We will break down the steps involved, including the information you ...

This chemistry video tutorial provides a basic introduction into internal energy, heat, and work as it relates to thermodynamics. It explains how to calculate the change in the internal energy of a gas as well as the PV work that occurs during gas compression or expansion. This tutorial also incorporates Boyle's law as it relatives to pressure ...

It is essential to calibrate Thermocouple regularly in order to attain precise readings. Calibration is the process used to maintain traceability of parameters with reference to set standards. In the following video we are demonstrating how a thermocouple is calibrated using comparison method. In Comparison Method, the accuracy of a test ...

How to calculate Melting temp., Mol Wt & GC content of oligonucleotides | Oligo calculation tool | Bioinformatics important tools and softwares

The Process 16 laboratory extruder is part of the well-known Thermo Scientific Process small-scale extruder series and maximizes your research efficiency and ROI. The throughput of the Process 16 extruder allows you to perform experiments efficiently and cost-effectively, even with small amounts of material, while saving valuable lab space ...

Melting curve analysis of DNA

Calibration levels are easily created directly in your injection list and automatically synchronized to the component table of your Processing Method.

Accessing the Relative Quantification application module and a summary walkthrough of how to utilize the module to analyze your data.

Explore Beamex Temperature calibrators: https://hubs.la/Q01j72Sl0 Learn how to calibrate and trim a HART RTD transmitter using a temperature calibrator. Michael from Beamex explains step-by-step how to calibrate an RTD temperature transmitter using the Beamex MC6, an advanced field calibrator and communicator. Which connection should you use in ...

Tutorial outlining Thermo Scientific extrusion screw assembly and elements for 16mm twin screw..

PRINCIPLES OF OPERATION: An extruder is a motor-driven screw inside a tube or barrel. Various raw materials, such as plastic compounds, are fed into the extruder. The outside of the barrel is a hollow jacket through which a hot fluid can be circulated in order to melt the material inside the barrel. The screw action moves the material through ...

In this episode I invited myself to a crash course in Thermo-Calc simulation software, as I wanted to learn more about the possibilities and the restrictions you meet when using it on everyday metallurgy. Nicholas Grundy from @ThermoCalcSoftware accepted the challenge and only needed a few input for his preparation. But there were a lot of ...

What is a thermocouple? A thermoelectric device for measuring temperature, consisting of two wires of different metals connected at two points, a voltage being developed between the two junctions in proportion to the temperature difference. Thanks for watching! Email: JumperManTech@Gmail.com Follow me on Instagram! @JumpRmantech

In this video, we are going to be introduce more properties and structure of polymer. Reference: Lamellae, spherulite and fibrillar structure--http://www.open.edu/openlearn/science-maths-technology/science/chemistry/introduction-polymers/content-section-5.6.1 Chewing gum--http://www.monitor.co.ug/Magazines/HealthLiving/Chewing-gum-more-harmful ...

Part review, part instruction. I recently purchased a Gdealer Instant Read Thermometer on Amazon for under $10. Let's see how it performs in some real-world metrology. The method I use here can be used to calibrate nearly any kitchen thermometer so check it out. As promised link to NIST ice point vid: https://www.youtube.com/watch?v=KYOJayWqB3g ...

Submit your Real-Time PCR questions and watch the rest of our videos at http://ow.ly/bQh0l. Life Technologies Sr. Field Application Specialist Doug Rains offers advices for fixing common software set-up mistakes when performing Real-Time PCR. I'm showing you a completed run file. Let's say I made a lot more mistakes than you did while setting ...

Thermoforming is a plastic molding technique, that results in a variety of highly usable products. - A plastic sheet is heated to a pliable temperature in an oven, it is stretched and formed in a mold and then cooled to a finished shape. - Thermoforming is grouped into two categories depending on the sheet thickness of the formed part. They are ...

Overview of heat exchanger analysis with set temperature boundary conditions. Dig it.