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In this video I show how to use Image J to measure color differences between samples. ImageJ download: https://imagej.nih.gov/ij/ ALESS Lab WebSite: http://ale.c.u-tokyo.ac.jp/support/aless-lab/ Diego's personal HomePage: https://dtvasques.wordpress.com/

Please click the links for the List of SOPs for Histopathology Lab: https://www.researchsop.com/2022/08/list-of-sops-and-documents-for.html In-vivo study protocols: https://www.researchsop.com/2022/04/List-of-All-SOPs-and-Documents-for-In-vivo-Laboratory.html How to Measure different Colored Areas in Images Using Image J Software My Recent ...

Learn how to use FIJI (ImageJ) to visualize the distribution of color in a 3D color space. The plugin used in this tutorial is Color Inspector 3D, which can be used with all image types. RGB values and frequency of each color pixel can be measured. The interactive plugin can also be used on an ROI in an image. Color Inspector 3D is also ...

You've carefully prepared your samples, labeling them with multiple fluorophores, and you've photographed each fluorophore with a colour camera. How do you put your images together into something you can publish?

Produced and created by Israel Estrada as part of the Advanced Chemical Communications CHM 5500 course taught by Dr. S. Chantal E. Stieber during Spring 2023 at Cal Poly Pomona. Supported by NSF CAREER # 1847926.

In this video you'll learn how to change the color of your microscope image color channels in Fiji (ImageJ). Adventures by A Himitsu https://soundcloud.com/a-himitsu Creative Commons — Attribution 3.0 Unported— CC BY 3.0 https://creativecommons.org/licenses/by/3.0/ Music provided by Audio Library https://youtu.be/MkNeIUgNPQ8

Important update (Jan 14, 2023): I have a new video that shows various techniques for better isolating specific colors in ***brightfield*** images in order to do more accurate comparisons. See here: https://youtu.be/O0LMhS_JtGs Otherwise, if you are just work with images acquired via fluorescence microscopy, this video should still suffice. You ...

Some examples of how to perform colocalisation using ImageJ plugins

Learn how to use FIJI (ImageJ) to segment/threshold RGB images like immunohistochemical (IHC) stains for subsequent analysis. Three methods are shown: RGB stacks and thresholding, Color Deconvolution, and Color Thresholding. See timeline below: #FIJI, #ImageJ, #RGB, #IHCstain 00:00 Introduction 00:09 RGB Color to RGB Stack 01:35 Color ...

Hi, This video shows how to change microscope image colours. It's always a good practice to select colour-blind friendly options. The steps in this video are also applicable to changing the colour of the time-lapse microscope series.

A look at contrast enhancement and histogram normalisation and equalisation in ImageJ

In this step-by-step tutorial, we’ll explore how to use ImageJ for image analysis. Whether you’re a beginner or looking to deepen your skills, this guide will walk you through the key features, from loading and processing images to analyzing and interpreting data. ImageJ is a powerful tool for scientific and medical imaging, and this ...

Quick guide to colocalization in Fiji/ImageJ. This was created as part of a lecture on Image Processing. Although the application is based on Fiji/ImageJ, it is also suitable for other software and applications. To download the Fiji software, please proceed here: https://imagej.net/Fiji/Downloads If you like this content please follow me on ...

In this ImageJ tutorial, an analysis of a bone superficial image is used to determine the trabecular thickness and bone percentage measurement in any defined area. A thresholding process is shown for that procedure. We offer this service through institutional channels at our lab. En este tutorial de ImageJ, se utiliza un análisis de una imagen ...

Basics of using ImageJ for biological image analysis. Topics covered: Multi-part TIFF file • Stitch together image stacks • RAM • Add labels and annotations • 8 bit, numbering conventions Minutewise stack • Measuring length, area, point manager • Color profile tool GFP and Phase, part 1 • Stack to images • Sub-stacks ...

Hi, Welcome back to my channel. This tutorial teaches you how to make an intensity plot for multiple fluorescence proteins. If you have a sample that is expressing more than a single fluorescence protein, this video will direct you step by step to generate the profile plot for merged fluorescence samples You can download the RGB profiler here ...

Learn how to use FIJI (ImageJ) to measure fluorescence intensity. This tutorial shows how measurements are affected when RGB images are used instead of the original multi-channel images. SUBSCRIBE to have first access to new video tutorials: https://www.youtube.com/@johanna.m.dela-cruz

The first of a series of ImageJ tutorials (an introduction to image analysis and using ImageJ). This first video covers the digital image and making measurements of object shape using thresholding and segmentation.

this video also list details of plugins available Link to download the plugins https://github.com/fabricecordelieres/IJ-Plugin_JACoP/releases Alternate download link https://imagej.net/ij/plugins/track/jacop2.html

Video Highlights and Helpful Links - The sample image, “C3-jw-30min 5_c5.tif”, used in this video can be found here (http://workshops.imagej.net/). - Thresholding (https://imagej.net/imaging/thresholding) involves choosing a cut-off value to delineate objects from non-objects/background. - Auto-Thresholding (https://imagej.net/plugins/auto ...

This video was created by University of Vermont student Jake Gamble as part of the 2020 Geocomputing class (Prof. Nico Perdrial)

This is the first in our lab's series of ImageJ Tutorials! This tutorial is all about measurements on diseased leaves! In this video we learn how to set scale for photographs! - 0:19 - Skip intro

This is an easy way to obtain counts for scientific images in the free program, Image J. You will learn computer aided image analysis so that you no longer manually count cells, particles, nuclei or any other biological structure in both color brightfield and fluorescence (including confocal). You will use the Find Maxima function in Image J to ...

Here's how to do a quick and easy selection in Affinity Photo, based on RGB colours. This is similar to Select Sampled Colour, but is based more on the primary colours and the range choice is made by Affinity. For related videos, see here: https://changingminds.org/disciplines/photography/affinity_photo/youtube_videos/050_selection_colour_range ...

Episode 2 in a series of ImageJ tutorials. This one covers intensity measurements using Regions of Interest (ROIs)

This video provides an overview on how to how to use Fiji (previously known as ImageJ) for basic image analysis. Conducting a size calibration, conducting a linear size measurement and creating a scale bar are included in this quick guide. This video comprises part of my student laboratory basics tutorial series, and aims to help students learn ...

This is the fourth in our lab's series of ImageJ Tutorials! In this tutorial, we will show how to perform manual and automatic count measurements. In this video we: 1 - 0:50 - Count germinated seed 2 - 5:40 - Count flowers and fruits 3 - 8:35 - Count leaves and measure their areas 4 - 12:53 - Count seed

This is the first in our lab's series of ImageJ Tutorials! This tutorial is all about measurements on diseased leaves! In this video we learn how to set scale for scanned images! - 0:18 - Skip intro

Learn how to use FIJI (ImageJ) to segment RGB (color or monochromatic) images, brightfield and other low-contrast images using the FIJI plugin Trainable Weka Segmentation. This plugin combines a collection of machine learning algorithms with a set of selected image features to produce pixel-based segmentations or classifications of biological ...

Things about ImageJ that I wish someone told me about (Macro and Batch Processing Features) #imagej (long title) A picture is worth a thousand words, and when you acquire an image for your research, be sure to consider every pixel as a potential source of data! My favorite software is certainly ImageJ because it is free, open-source, and has ...

Watch this video to find out about changing lookup tables for your multi-channel images in ImageJ. For more help, see our website at igmmimaging.com

Video Highlights and Helpful Links - The sample image, “C3-jw-30min 5_c5.tif”, used in this video can be found here (http://workshops.imagej.net/). **Note: The names ‘ImageJ’ and ‘Fiji’ are used interchangeably… we recommend new users download/install/use the Fiji distribution (https://imagej.net/software/fiji/index) of ImageJ, as ...

This Tutorial is all about how to quickly Edit & Label PCR Gel Image Using ImageJ software. Presented by - Elvis Samuel Join Our Telegram Channel for free Softwares, Notes, and References books: JUST BIOTECH GEEKS: https://t.me/justbiotechgeeks #Gel_electrophoresis#Biotechnology#Research#Science INTRODUCTION TO GEL ELECTROPHORESIS : https ...

Publication: EzColocalization: An ImageJ plugin for visualizing and measuring colocalization in cells and organisms https://www.nature.com/articles/s41598-018-33592-8 Github: https://github.com/DrHanLim/EzColocalization Narrator: Weston Stauffer

Learn how to use FIJI (ImageJ) to select and measure objects or ROIs in a 2-dimensional image using the ROI Manager. Additional features of the ROI Manager will be demonstrated. See chapter timeline below. #FIJI, #ImageJ, #ROI, #ROIManager Chapters: 00:00 Introduction 00:06 Selecting objects with drawing tools 00:56 Using the wand tool to ...

Basics of working with ImageJ Grain size and Threshold adjustment

Learn how to display images in color with FIJI (ImageJ). #FIJI, #ImageJ, #color, #mergechannels, #LUT, #RGB, #2021 SUBSCRIBE to have first access to new video tutorials: https://www.youtube.com/channel/UCFKPbB-h1GbuOG9gwD1tXEA

This video will demonstrate a method that allows semi-automated quantification of hair cells in the entire utricle by staining adult utricle cultures for Pou4f3, a hair cell-specific transcription factor. This video will use confocal images of control, neomycin-treated, and cisplatin-treated utricles to show how ImageJ can be utilized for the ...

For quantification of IHC images using imageJ, please watch this video. https://www.youtube.com/watch?v=gxnWYhZ0z4k&t=620s This video lecture describes how to quantify western blot using imagej. 1. How to upload western blot image in imagej 2. How to invert image in imagej 3. How to adjust background/ brightness and contrast of western blot ...

A tutorial on how to select by color and color an entire selection in Photoshop cc.

Just a simple step on how to put a label or anything desired into the SEM tiff file. With the help of open source Image J

A brief introduction to simple image analysis functions in ImageJ/Fiji. This is a recording of a Zoom meeting led by HCBI Director of Imaging Doug Richardson.

In this video, I share a macro (available here: https://doi.org/10.5281/zenodo.5595203) that automates the extraction of summary statistics from channels of three colorspaces (RGB, Lab, and HSB). This macro functions to 1) open an image from a directory folder, 2) threshold the image to segment out the object of interest from the background ...

A screencast tutorial demonstrating four methods to convert an RGB image into 8-bit grayscale

A viewer contacted me about a situation that seemed a bit different from doing color intensity measurement on a typical color image in ImageJ/FIJI. If you have a combined image where brightfield (or phase contrast or DIC) and a fluorescence channel are merged together, and if you do *not* have the separate original images/channels, you can do ...

In this video we will talk about annotation of an image with the help of text or arrows in imageJ/ Fiji software. The process is simple. The steps included are: 1. Selection of text Tool 2. Text writing at the desired location on image 3. Editing of the text 4. Saving the text by Draw function 5. Drawing the arrow (arrow annotation) / add arrow ...

In this episode of the Photoshop Training Hour, you will learn 5 tricks to select specific colors in Photoshop! This live stream is sponsored by MSI! Photoshop Short Tutorials: https://www.youtube.com/playlist?list=PL3bfN-31F9Rf__SKNBU2NfhBbx1zyxs4B Creator Z16P Learn More: https://msi.gm/CreatorPTC Buy Now: https://msi.gm/Creatorz16pNE • MSI ...