(A) The cisternal maturation model of protein movement through the Golgi. As a new cis cisterna is formed it traverses the Golgi stack, changing as it matures by accumulating medial, then trans ...
The mechanisms for cargo transport through the Golgi complex have been the subject of a long-lasting debate [1].Many reviews have addressed the details of these different models [2, 3, 4].The most popular models include cisternal maturation [5], vesicular transport between stable compartments [6] and the rapid partitioning model [7].Each of these models possesses strengths and weaknesses, and ...
A variety of secretory cargoes move through the Golgi, but the pathways and mechanisms of this traffic are still being debated. Here, we evaluate the strengths and weaknesses of five current models for Golgi traffic: (1) anterograde vesicular transport between stable compartments, (2) cisternal prog …
The model, shown in Figure 1 and fully described in the Appendix 1 Detailed model is a coarse-grained representation of the Golgi Apparatus. The parameters used in the model are summarised in Appendix 1—table 1, and the quantities used to charaterise a compartment and the steady-state of the system are summarised in Appendix 1—table 2 and Appendix 1—table 3, respectively.
Most of these models are not able to explain recent data on the evolution of genes involved in intracellular transport and Golgi evolution. The carrier maturation model proposing that fusion of the large cargo domain with the distal (closer to the plasmalemma) compartment precedes fission of the domain from the proximal compartment exhibits the ...
The vesicular trasnport model posits that the Golgi cisternae are stable compartments that house certain protein modification enzymes that function to add or remove sugars, add sulfate groups, and ...
The Golgi apparatus processes proteins received from the endoplasmic reticulum (ER) through vesicular transport.Proteins are modified via glycosylation, including N-linked and O-linked types, crucial for stability and function.The Golgi consists of cisternae organized into cis, medial, and trans sections, facilitating protein maturation. Proteins can move through the Golgi via the vesicular ...
Scheme of intra-Golgi transport according to the CMPM. The main postulate of this model is that during intra-Golgi transport, the amount of cargo inside the cisterna during its progression is not changed, and that COPI vesicles (COPI, black dots; Golgi-resident proteins, colored dots) should be concentrated in COPI vesicles.
The essence of different models of intra-Golgi transport and their problems are described in detail by Mironov and Beznoussenko . The progression model (or the concept of cis-to-trans flow) was the first mechanism proposed for the explanation of mechanisms of intracellular transport.
The carrier maturation model of intra-Golgi transport. The fusion of large TCs with the distal compartment occurs before its fission from the proximal one. The necessity for the retrograde dissociative carriers (i.e., vesicles) containing markers of the distal compartment is eliminated. 1: The cargo-containing, ER-to-Golgi carrier (EGC; colored ...
The Golgi complex is the central sorting and processing station of the secretory pathway, ensuring that cargo proteins, which are synthesized in the endoplasmic reticulum, are properly glycosylated and packaged into carriers for transport to their final destinations. ... Although only a proof in principle, this model for the first time invokes ...
The rapid-partitioning model furthermore accounts for other Golgi characteristics. With the model parameters that produce the timing of the cargo wave, cargo preferentially enriches in the cis-most cisterna during the first 3 min of cargo entry into the Golgi after the usual 40°C to 32°C temperature shift , which is also observed in a ...
Recently, we proposed the kiss-and-run (KAR) model of intra-Golgi transport (Mironov and Beznoussenko in Int J Mol Sci 13(6):6800-6819, 2012), which can be symmetric, when fusion and fission occur in the same location, and asymmetric, when fusion and fission take place at different sites. Here, we compare the ability of main models of the intra ...
Directionality of intra-Golgi vesicles is still a matter of debate and will not be discussed here (for a discussion on models of intra-Golgi trafficking, please see Ito et al., 2014; Mironov and ...
The vesicular model, compartment (or cisterna) maturation model, and the diffusion model compete with each other for the right to be the paradigm within the field of the intra-Golgi transport.
