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The Gram stain is a crucial differential staining technique used in microbiology to classify bacteria into two major groups: Gram-positive and Gram-negative. This classification is based on the differences in cell wall compositions of the bacteria, which affect how they retain the crystal violet dye during the staining process.
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1. Heat fix bacterial smear 2. Cover with Crystal Violet (Primary Stain) and wait 60 seconds 3. Rinse slide with distilled water until stain is removed 4. Cover with Gram's Iodine (Mordant) and wait for 60 seconds 5. Rinse with distilled water 6. 2-3 drops of Decolorizer (95% Ethanol), or until color is clear, rinse with distilled water 7. Cover with Safranin (Counter Stain) and wait 45 seconds 8.
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Microbiology Exam 1 (Chapters 1, 3-5) 4 (10 reviews) Terms in this set (314) Microbiology. Specialized area of biology that deals with organisms ordinarily too small to be seen without magnification.
Microbiology Lab Practical #1 Flashcards - Quizlet
- dangerous and exotic microorganisms with high risk of aerosol transmitted infections - rarely have treatments or vaccines for them and the disease are often fatal - change clothing before entering lab - shower upon exit - decomanination of everything after lab - positive pressure suit must be worn - negative air flow within the building - lab ...
Microbiology Practical #1 Flashcards - Quizlet
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Microbiology Practical 1 Flashcards - Quizlet
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