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Double click on any line tool to specify the line width by opening the Image Adjust Line Width…↓ widget, on which is also possible to apply a cubic spline fit to a polyline selection. Check the Sub-pixel resolution checkbox in Edit Options Profile Plot Options… ↓ to create line selections with floating-point coordinates ( see Selections ...

Hi, The slope of a trace on a kymograph or spatio-temporal diagram gives the speed of the object that produced the trace (for more explanations also see this page on imagej.net).When I asked on the ImageJ mailing list whether measuring this slope could be made more convenient, @imagejan and Herbie Gluender sent nice macro code that provides a line-selection type tool that shows custom ...

Tools. Area Selection Tools Use these tools to create area selections that will be operated on separately from the rest of the image. The contents of an area selection can be copied to the internal clipboard, cleared (to white), filled with the current drawing color, outlined (using Edit/Draw), filtered, or measured.Use the backspace key as a shortcut for Edit/Clear.

Use the straight line selection tool to select a vertical line down the center of the third lane from the left. Choose Analyze > Plot Profile. Double-click the straight line selection tool button on the ImageJ toolbar to open the Line Width dialog box, and set the line width to 20. Activate the gel.gif image window and make another profile plot.

All ImageJ tools share common features . Tool bar. The on the bottom right corner of some icons in the toolbar depicts a contextual menu that can be accessed by right-clicking on the tool icon (e.g., Stacks Menu↓). If an ‘Options ... Line selection tool. Use these tools to create line selections. The three line selection tools share the ...

This is always true in ImageJ for tools showing a small red arrow at the bottom right of the tool button. Right-clicking on the tool shows a menu with the similar available tools. Select the straight line tool and draw a line by clicking anywhere on the image and moving the mouse without releasing the mouse button until you want to end your line.

Line Selection Tools - Use these tools to create straight, freehand or segmented line selections. A pop-up menu (note the little triangle) is used to select one of three line types. The pop-up menu is accessed by clicking on the line selection tool's icon and holding the mouse button down for at least 1/2 second. Once you have created a line ...

In general (see Area Selection Tools↓ and Line Selection Tools↓ for details), selections are resized by dragging one of the selection handlers. While dragging, holding Ctrl resizes the selection around its center, holding Alt imposes a fixed aspect ratio and holding Shift forces a 1:1 aspect ratio.

The selection tool line for creating ROIs used to have a yellow color, which was pretty practical, especially for working with fluorescent microscopy and black background. However, after some “great” updates, it changed color to black (making it practically useless for me).

Hi. I am using the segmented line tool in ImageJ to count circuli on fish scale images. One problem I am running into is that it is easy to loose the selection once i have completed the line. Any click next to the line will reset it and remove all the previous work. Is there a setting or option somewhere to disable that behavior of the software? Maybe requring a double click (instead of a ...

A community for the discussion of image analysis, primarily using ImageJ (and FIJI), a free, open source, scientific image processing and analysis program using Java, and is used worldwide, by a broad range of scientists. ... Do mean a line drawn by hand with the line-selection tool? If yes, click this tool in the toolbar, then set the cursor ...

How to select a line in an image using ImageJ/Fiji by setting its start and end points? As in not drawing it over the image, but inputting the coordinates directly somewhere. imagej; fiji; Share. Improve this question. ... Fiji/ImageJ - Measure tool when x and y resolution of image not equal. 0.

1. Before using this command, use the straight line selection tool to make a line selection that corresponds to known distance. 2. Then, bring up the Set Scale dialog, enter the known distance and unit of measurement, then click OK. ImageJ will have automatically filled in the Distance in Pixels field based on the length of the line selection.

Before using this command, use the straight line selection tool to make a line selection that corresponds to known distance. Then, bring up the Set Scale dialog, enter the known distance and unit of measurement, then click OK. ImageJ will have automatically filled in the Distance in Pixels field based on the length of the line selection.

The curved object must first be outlined using the Segmented Line Selection Tool↑. Double click on the line tool icon to open the Image ... It also prevents multiple instances when running ImageJ from the command line. Note that you may get a security alert the first time ImageJ starts with this option enabled.

Hi All, when I convert a polyline or freeline selection to an area using “Edit > Selection > Line to Area” the resulting selection seems to be translated by -1 (or -2 ) pixels in x and y: The simplest way to replicate this is to create a blank image of 50,50 Select the “freehand line” or “segmented line” selection tool. Make a selection that is mostly diagonal (easier to see the ...

The numeric codes for those tools were revealed in the macro recording window as setTool(4) and setTool(18). Use the record macro function to see what ImageJ calls internally when you click on a tool button. Clicking on the Straight Line tool and the Paintbrush tool showed the two commands ImageJ issued to select those tools.

ImageJ has a simple and effective tool for Straightening images of curved structures, ... First, select the “Segmented Line” selection tool by right-clicking on the “Straight Line Selections” tool icon in the toolbar and choosing the “Segemented Lines” option. Then click along a selection of points along the structure, completing ...

Hey guys, for a specfic use-cas in my image analysis routine it would be nice to be able to modify the line-tool in imagej / fiji. I need to do alot of line measurements perpendicular to a certain structure. Therefore it would be nice to have a perpendicular baseline in the line measurement tool as orientation.