Changes in chromatin architecture induced by epigenetic mechanisms are essential for normal cellular processes such as gene expression, DNA repair, and cellular division. Compact chromatin presents a barrier to these processes and is highly regulated by epigenetic markers binding to components of the nucleosome. Histone modifications directly influence chromatin dynamics and facilitate ...
Subsequent genetic studies defined a broader role for FACT in maintaining chromatin architecture. Loss of FACT function can lead to a variety of chromatin abnormalities, including (1) loss or disorganization of nucleosomes and mislocalization of the yeast histone H2A.Z in transcribed regions (Feng et al., 2016; Jamai et al., 2009; Jeronimo et ...
Histone chaperone FACT FAcilitates Chromatin Transcription: mechanistic and structural insights. Author links open overlay panel Keda Zhou 1, Yang Liu 1 2, Karolin Luger 1 2. Show more. Add to Mendeley. Share. ... [27 ••] share an overall architecture but differ in the presence of a second H2A-H2B dimer that is contacted by SSRP1 (Figure 3 ...
Establishment and maintenance of chromatin architecture are promoted independently of transcription by the histone chaperone FACT and H3-K56 acetylation in Saccharomyces cerevisiae. Genetics 211, 877–892. 10.1534/genetics.118.301853. [PMC free article] [Google Scholar] 41. Erkina TY, and Erkine A (2015). ASF1 and the SWI/SNF complex interact ...
The results are consistent with emerging models that emphasize FACT's importance in establishing global chromatin architecture prior to transcription, promoting transitions among different states as transcription profiles change, and restoring chromatin integrity after it is disturbed.
Histone chaperone FACT (facilitates chromatin transcription) is well known to promote chromatin recovery during transcription. However, the mechanism how FACT regulates genome-wide chromatin accessibility and transcription factor binding has not been fully elucidated. Through loss-of-function studies, we show here that FACT component Ssrp1 is required for DNA replication and DNA damage repair ...
RNA Pol II transcription is supported by chromatin remodelers and histone chaperones. The histone chaperone facilitates chromatin transcription (FACT) was identified and purified from nuclear extracts as a factor that stimulates transcription elongation on a chromatinized DNA template in vitro. 6 The human FACT complex is composed of two subunits: suppressor of Ty 16 (SPT16) and structure ...
Therefore, the promoter architecture of the cac1∆ rtt106∆ mutant is likely first reconstructed to prime active transcription and restore chromatin in the gene body as proposed for chromatin ...
Facilitates chromatin transcription (FACT) is a histone chaperone that supports transcription through chromatin in vitro, but its functional roles in vivo remain unclear. ... FACT maintains chromatin architecture and thereby stimulates RNA polymerase II pausing during transcription in vivo Mol Cell. 2024 Jun 6;84(11):2053-2069.e9. doi: 10.1016 ...
INTRODUCTION. FACT (FAcilitates Chromatin Transcription) is a broadly conserved histone chaperone that was named for its ability to promote elongation by RNA Pol II through nucleosomes in vitro ().However, FACT appears to function in at least two fundamental processes, as it associates with transcription machinery but it also directly contacts DNA replication complex components, including the ...
Structural and functional analyses of RNA polymerase II−nucleosome complexes reveal how the chromatin remodeler Chd1 and the histone chaperone FACT mediate Pol II transcription through a nucleosome.
The human FACT (facilitates chromatin transcription) complex, composed of two subunits SPT16 (Suppressor of Ty 16) and SSRP1 (Structure-specific recognition protein-1), plays essential roles in nucleosome remodeling. However, the molecular mechanism of FACT reorganizing the nucleosome still remains elusive. In this study, we demonstrate that ...
FACT is a roughly 180 kDa heterodimeric protein complex important for managing the properties of chromatin in eukaryotic cells. Chromatin is a repressive barrier that plays an important role in protecting genomic DNA and regulating access to it. ...
To examine whether FACT is positioned to regulate chromatin structure during transcription in vivo, we performed comparative immunofluorescence analysis on Drosophila polytene chromosomes. Immunofluorescence staining reveals the global distribution of FACT (Spt16) relative to the hyperphosphorylated, elongating Pol IIo ().Antibodies against either FACT or Pol IIo label many sites on ...
The histone chaperone FACT (FAcilitates Chromatin Transcription) modulates nucleosome structure during many processes requiring access to chromatinized DNA. Here, we review recent biochemical and structural insight into how FACT acts in both nucleosome assembly and disassembly, to maintain chromatin structure while DNA is being worked on by ...
