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FACT (fa cilitates c hromatin t ranscription, [1] sometimes fa cilitates c hromatin t ransactions [2][3]) is a heterodimeric protein complex that affects eukaryotic RNA polymerase II (Pol II) transcription elongation both in vitro and in vivo.

The histone chaperone facilitates chromatin transcription (FACT) was identified and purified from nuclear extracts as a factor that stimulates transcription elongation on a chromatinized DNA template in vitro. 6 The human FACT complex is composed of two subunits: suppressor of Ty 16 (SPT16) and structure-specific recognition protein 1 (SSRP1 ...

The facilitates chromatin transcription (FACT) complex is involved in chromatin remodeling during transcription, replication, and DNA repair. FACT was previously considered to be ubiquitously expressed and not associated with any disease. However, we discovered that FACT is the target of a class of …

The FAcilitates Chromatin Transcription (FACT) complex was originally discovered as a factor that facilitates transcription elongation through nucleosomes in vitro [1]. The FACT complex is composed of two subunits, SPT16 (Suppressor of Ty 16) and SSRP1 (Structure Specific Recognition Protein 1) [2].

Facilitates chromatin transcription (FACT) is an important histone chaperone that plays crucial roles during various cellular processes. Here, we analyze the structural characteristics of FACT, discuss how FACT regulates nucleosome/chromatin reorganization and summarize possible functions of FACT in transcription, replication, and DNA repair.

The histone chaperone FACT (FAcilitates Chromatin Transcription) modulates nucleosome structure during many processes requiring access to chromatinized DNA. Here, we review recent biochemical and structural insight into how FACT acts in both nucleosome assembly and disassembly, to maintain chromatin structure while DNA is being worked on by polymerases.

The human FACT (facilitates chromatin transcription) complex, composed of two subunits SPT16 (Suppressor of Ty 16) and SSRP1 (Structure-specific recognition protein-1), plays essential roles in nucleosome remodeling. However, the molecular mechanism of FACT reorganizing the nucleosome still remains elusive.

FACT also associates with RNA Pol III complexes, is present at the chromatin of genes transcribed by Pol III and facilitates their transcription in cells. Our findings indicate that, beyond the established role in Pol II transcription, FACT has physiological functions in chromatin transcription by all three nuclear RNA Pols.

Ordered nucleosome disassembly and reassembly are required for eukaryotic DNA replication. The facilitates chromatin transcription (FACT) complex, a histone chaperone comprising Spt16 and SSRP1, is involved in DNA replication as well as transcription. ...

INTRODUCTION FACT (FAcilitates Chromatin Transcription) is a broadly conserved histone chaperone that was named for its ability to promote elongation by RNA Pol II through nucleosomes in vitro (1). However, FACT appears to function in at least two fundamental processes, as it associates with transcription machinery but it also directly contacts DNA replication complex components, including the ...

FACT (facilitates chromatin transcription) is an essential histone chaperone consisting of two subunits, Spt16 (suppressor of Ty homolog 16) and Ssrp1 (structure-specific recognition protein 1) (3), both of which are highly conserved in eukaryotes (4, 5).

The FACT (facilitates chromatin transcription) complex is required for transcript elongation through nucleosomes by RNA polymerase II (Pol II) in vitro. Here, we show that FACT facilitates Pol II–driven transcription by destabilizing nucleosomal structure so that one histone H2A-H2B dimer is removed during enzyme passage.

We developed a highly efficient strategy, Perturb-multiome, to simultaneously profile chromatin accessibility and gene expression in single cells with CRISPR-mediated perturbation of master transcription factors (TFs).

Additionally, LLPS indirectly influences the three-dimensional organisation of chromatin by modulating interactions between chromatin and key nucleosomes [26]. HLBs are BMCs that form at replication-dependent (RD) histone genes within animal cells, playing a crucial role in regulating the transcription and processing of histone mRNAs.

These modifications influence gene transcription by altering chromatin structure and gene accessibility, thereby precisely regulating cellular functions and phenotypes in living organisms.

The histone chaperone FACT (FAcilitates Chromatin Transcription) modulates nucleosome structure during many processes requiring access to chromatinized DNA. Here, we review recent biochemical and structural insight into how FACT acts in both nucleosome assembly and disassembly, to maintain chromatin …

HIV-1 can establish a lifelong infection by incorporating its proviral DNA into the host genome. Once integrated, the virus can either remain dormant or start active transcription, a process governed by the HIV Tat protein, host transcription factors and the chromatin landscape at the integration site. Histone-modifying enzymes and chromatin-remodeling enzymes play crucial roles in regulating ...

The development of hematopoietic cell lineages is a highly complex process governed by a delicate interplay of various transcription factors. The expression of these factors is influenced, in part, by epigenetic signatures that define each stage of cell differentiation. In particular, the formation of B lymphocytes depends on the sequential silencing of stemness genes and the balanced ...

The facilitates chromatin transcription (FACT) complex, a histone chaperone comprising Spt16 and SSRP1, is involved in DNA replication as well as transcription.

Background During meiosis, the mammalian genome is organised within chromatin loops, which facilitate synapsis, crossing over and chromosome segregation, setting the stage for recombination events and the generation of genetic diversity. Chromatin looping is thought to play a major role in the establishment of cross overs during prophase I of meiosis, in diploid early primary spermatocytes ...