mavii

Global transcription inhibition stabilizes CTCF clusters (Gu et al, 2020), and several non‐coding RNAs have been shown to destabilize the interaction between CTCF and DNA, which leads to loss of CTCF–CTCF loops (Sun et al, 2013; Oh et al, 2021a). Together, these data indicate that the non‐coding RNA portfolio in the cell might constitute ...

In this regard, we now report two independent features that disrupt CTCF association with chromatin: inhibition of transcription and disruption of CTCF-RNA interactions through mutations of 2 of its 11 zinc fingers that are not required for CTCF binding to its cognate DNA site: zinc finger 1 (ZF1) or zinc finger 10 (ZF10).

Inhibition of transcription is mediated by the central ZF domain and by sequences both N-terminal and C-terminal to the ZF region 6, 17. Repression by the N ... the thyroid hormone receptor (T3R) modulates the effects of CTCF on transcription. In the case of the chicken lysozyme silencer, synergistic repression of the adjacent CTCF and T3R ...

During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the architectural factor CTCF and the process of transcription to genome re-sculpting in newborn nuclei. Deple …

A fraction of CTCF clusters, enriched for those with ≥3 molecules, are coupled with cohesin complexes with a characteristic physical distance suggestive of a defined molecular interaction. Acute degradation of the cohesin unloader WAPL or transcriptional inhibition (TI) result in increased CTCF clustering.

CCCTC-binding factor (CTCF) plays indispensable roles in transcriptional inhibition/activation, insulation, gene imprinting, and regulation of 3Dchromatin structure. CTCF contributes to formation of genome multi-dimensions, regulation of dimensional changes, or control of central signals to transcri …

However, a clear mechanistic insight into selective inhibition of CTCF by iAs requires a detailed genome-wide functional analysis of CTCF binding in iAs-T cells. Future studies will help delineate such selectivity. Interestingly, other heavy metals have also been shown to inhibit CTCF binding at weaker binding sites (Jose et al., 2014). Whether ...

Maintenance of CTCF- and Transcription Factor-Mediated Interactions from the Gametes to the Early Mouse Embryo. Yoon Hee Jung 1 ∙ Isaac Kremsky 1 ∙ Hannah B. Gold 1 ∙ ... persistent sites with bound TFs inherited from gametes and retained through early embryogenesis may inhibit remethylation at their binding motifs. These sites may then ...

Transcription inhibition. G1E-ER4 CTCF-AID-mCherry cells expressing OsTiR-IRES-GFP were arrested in prometaphase with nocodazole (200 ng/ml) as above. Triptolide (1 μM) was added to the cultures during the last hour of nocodazole exposure. For “+auxin” samples, auxin was added during the last 4 h of nocodazole treatment. ...

Further study showed that HN1 was negatively correlated with CTCF in an independent thyroid-cancer cohort, and inhibition of HN1 enhanced the expression of CTCF in ATC cells. Overexpression of CTCF significantly reversed the dedifferentiation phenotypes of ATC cells, whereas simultaneously inhibiting HN1 and CTCF was unable to recover the level ...

The degree of CTCF-binding reduction upon auxin treatment and the associated gains in uasTrx were only weakly correlated (Extended Data Fig. 1i), suggesting a nonlinear relationship between CTCF occupancy and uasTrx inhibition. Because strong CTCF-bound sites (CBSs) tend to be conserved across cell types 19,27, we assessed CTCF occupancy across ...

CTCF also promotes transcription activation directly at gene promoters or via fostering of contact with distal enhancers. ... Overall, there were few significant changes in intragenic CTCF binding after transcription inhibition with 5,6-dichloro-1–D-ribofuranosyl-benzimidazole (DRB) (5 h; 11 gains/0 loss; 9,134 sites total; ...

The chromatin organizing proteins CTCF and cohesin are known to exhibit spatial heterogeneity, forming “clusters” inside the nucleus. Here, Gu, Comerci, McCarthy, et al. use super-resolution microscopy to measure quantitative parameters governing CTCF and cohesin cluster formation and demonstrate that cohesin and transcription play opposing roles in CTCF clustering.

CTCF has it all. The transcription factor binds to tens of thousands of genomic sites, some tissue-specific, others ultra-conserved. It can act as a transcriptional activator, repressor and insulator, and it can pause transcription. CTCF binds at chromatin domain boundaries, at enhancers and gene promoters, and inside gene bodies.

Together, the evidence points to CTCF repressing uasTrx transcription through initiation inhibition rather than Pol II stalling, which is consistent with our recent observation that the presence ...

For example, CTCF activates the transcription of a subset of the promoters it binds 4, ... how to abrogate compartmentalization is currently unknown. Inhibition of transcription, ...

ZHX2 interacts with CTCF to activate gene transcription in hypoxia (A) Bar plot showing number of ATAC-seq peaks in T47D cells infected with shCtrl or shZHX2 under hypoxia. ... S625A or S628A mutations could partially inhibit ZHX2-CTCF interaction under hypoxia (Figure S7 I); the double mutant S625A/S628A (SA) completely blocked this ...

CTCF has it all. The transcription factor binds to tens of thousands of genomic sites, some tissue-specific, others ultra-conserved. It can act as a transcriptional activator, repressor and insulator, and it can pause transcription. CTCF binds at chromatin domain boundaries, at enhancers and gene pr …

The growth inhibition induced by wild-type CTCF was markedly relieved as a consequence of these mutations 7. Regulation of CKII by the p38α mitogen-activiated protein kinase (MAPK) ... Molecular weight abnormalities of the CTCF transcription factor: CTCF migrates aberrantly in SDS–PAGE and the size of the expressed protein is affected by the ...