mavii

Higher-order chromatin structure is temporarily disrupted during mitosis. Here the authors show that loss of the architectural factor CTCF results in failure to form structural loops and leads to ...

During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the architectural factor CTCF and the process of transcription to genome re-sculpting in newborn nuclei. Deple …

CTCF is required for the proper re-formation of contacts among regulatory elements and normal transcription reactivation.

Collectively, these findings indicate that IFN−γ induces a rapid reconfiguration of the chromatin in the MHC at CTCF binding sites to poise the region for subsequent transcriptional activation.

Recent reports have suggested that CTCF binding is more dynamic during development than previously appreciated. Here, we set out to understand the extent to which shifts in genome-wide CTCF occupancy contribute to the 3D reconfiguration of fine-scale chromatin folding during early neural lineage commitment.

The focus on the de novo formation of nuclear architecture during G1 entry yields insights into the contributions of CTCF and transcription to chromatin architecture dynamics during the mitosis to ...

We demonstrate that CTCF loss alters short-range compartmental interactions after mitosis, suggesting a previously underappreciated role for CTCF in genome compartmentalization. CTCF is required for the proper re-formation of contacts among regulatory elements and normal transcription reactivation.

In this study, we show that Ctcf expression increases gradually during reprogramming, which is functionally required for induced pluripotency. CTCF binds and represses somatic genes as an insulator in the early phases of reprogramming and helps maintain chromatin accessibility and partial EP interactions for pluripotency genes.

CTCF is an architectural protein with a critical role in connecting higher-order chromatin folding in pluripotent stem cells. Recent reports have suggested that CTCF binding is more dynamic during development than previously appreciated. Here, we set out to understand the extent to which shifts in genome-wide CTCF occupancy contribute to the 3D reconfiguration of fine-scale chromatin folding ...

Recent studies have implicated that CTCF occupies discrete positions on the X-chromosome and plays a distinct and pivotal role in the orchestration of XCI including X-chromosome counting, pairing, choice, structural reconfiguration of inactive X, tethering the inactive X to specific nuclear compartments, maintenance of X-inactivation and ...

During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. Here we exploited the M- to G1-phase progression to interrogate the contributions of the architectural factor CTCF and the process of transcription to re-sculpting the genome in newborn nuclei. Depletion of CTCF specifically during the M- to G1-phase transition altered the re ...

This is notably the case at the promoter of the DNR-induced NFKB2 gene. DNR leads to a reconfiguration of chromatin loops engaging CTCF- and SUMO-bound NFKB2 promoter with a distal cis-regulatory region and inhibition of SUMOylation with ML-792 prevents these changes.

During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the architectural factor CTCF and the process of transcription to genome re-sculpting in newborn nuclei. Depletion of CTCF during the M- to G1-phase transition alters short-range compartmentalization after ...

Given that epigenomic reprogramming and reconfiguration of chromatin architecture are essential during differentiation and development 53, 64 – 67, our results suggest that differential H3K9me3 regulates cell/tissue-type specific CTCF binding and describe an addition layer of establishing different chromatin structures between cellular ...

Higher-order chromatin structure is temporarily disrupted during mitosis. Here the authors show that loss of the architectural factor CTCF results in failure to form structural loops and leads to inappropriate cis-regulatory contacts and alterations of compartmental interactions after mitosis. Furthermore, they show global 3D architecture is set up without transcription, but that transcription ...

Among them, the CCCTC-binding factor CTCF is highly enriched at SUMO-binding sites found in cis -regulatory regions. This is notably the case at the promoter of the DNR-induced NFKB2 gene. Its induction is preceded by a SUMO-dependent reconfiguration of chromatin loops engaging its CTCF- and SUMO-bound promoter with distal cis -regulatory regions.

During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the architectural factor CTCF and the process of transcription to genome re-sculpting in newborn nuclei. Depletion of CTCF during the M- to G1-phase transition alters short-range compartmentalization after ...

Abstract During mitosis, transcription is globally attenuated and chromatin architecture is dramatically reconfigured. We exploited the M- to G1-phase progression to interrogate the contributions of the architectural factor CTCF and the process of transcription to genome re-sculpting in newborn nuclei. Depletion of CTCF during the M- to G1-phase transition alters short-range ...

Abstract CTCF is an architectural protein with a critical role in connecting higher-order chromatin folding in pluripotent stem cells. Recent reports have suggested that CTCF binding is more dynamic during development than previously appreciated. Here we set out to understand the extent to which shifts in genome-wide CTCF occupancy contribute to the 3-D reconfiguration of fine-scale chromatin ...

It is shown that loss of the architectural factor CTCF results in failure to form structural loops and leads to inappropriate cis-regulatory contacts and alterations of compartmental interactions after mitosis, and global 3D architecture is set up without transcription, but that transcription contributes to proper gene domain formation. During mitosis, transcription is globally attenuated and ...